They can then load data acquired on a Beckman Coulter Cyan and have it scaled a different way.įurthermore, with the new cytometer scaling preferences, the user can manipulate the preference sets for each cytometer. This way, a user can load data acquired on a BD instrument running FACSDiva and have it scaled one way (similar to FACSDiva). In addition, a preference set is generated for the data from all the different cytometers that have been opened in FlowJo. Default scaling preferences are then loaded into the preference pane based on the value of the $CYT keyword. In version 10, the cytometer preferences are set by reading the cytometer ($CYT) keyword from the files upon load. Hence, scaling did not automatically change based on the cytometer from which the data were acquired. In older versions of FlowJo, the scaling was limited to number of decades and general transform settings globally.
#Flowjo flow cytometry software
Hence, the software can control the scaling and transformation upon load. All FCS 3.1 data is written as linear, untransformed and uncompensated data. However, with FCS 3.1, the digital standard, scaling and display of data is controlled by the software in which it is analyzed. FCS 2.0 data is considered the analog standard and scaling is controlled by the file. However, these can be changed or refined. For more information about FlowJo’s display of data for specific cytometers, please see FlowJo and Your Cytometer.ĭata is written in two common standards, FCS 2.0 and FCS 3.1 (previously FCS 3.0, Data File Standard for Flow Cytometry, Version FCS 3.1, Normative Reference). We have a number of default settings for the various cytometers set.
FlowJo version 10 allows you to set up scaling preferences based on the specific cytometer being used.
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